When he disappears, the long anaconda escapes from the cage and kills Peter in a mine filled with blood orchids.

Peter 'J.D.' Murdoch, a billionaire suffering from bone cancer, sends his assassin Eugene and his team of henchmen mercenaries to find Peter and the serum Peter created so that it can cure him. Amanda Hayes, the sole survivor of the previous snake attack, and two officers also go in search of Peter, determined to destroy the serum and kill the snake.

On the way, they meet Alex, a trekker who appears lost in the Romanian Carpathians, while doing a paleopathology project. When they discover the blood orchids in the mine, Amanda sets the explosives to destroy the orchids, but she and the group are attacked by the anaconda that kills one of the officers with her while the other officer hides and disappears. Amanda is able to trick the snake into causing a cave in that 'kills' the snake however as she and Alex attempt to leave the mine, she is knocked unconscious and Alex escapes to get more help.

Meanwhile, a group of explorers: Jackson, girlfriend Wendy, Wendy's best friend Heather, and brothers Patrick and Scott, get dragged into the search for the snake. Due to the serum, the anaconda can no longer die without significant damage to its internal organs and regenerates itself from the previous cavein. The next day, The group Murdoch hires is attacked by the anaconda and it kills one of them. Alex and Amanda are able to regroup after Amanda saves Alex from the snake and they both eventually find the explorers from earlier after the snake attacks and tears off the arm of Patrick. Eugene and his henchmen eventually find Hayes and Jackson's group and captures them. When Amanda is uncooperative in their efforts to get information about the serum Eugene shoots and kills Patrick. During this moment of confusion Wendy tries to escape and Eugene shoots her dead which enrages Jackson.ДЄmanda and Scott are forced to find the serum, and are accompanied by two henchmen of Eugene to find it. There the two find the serum but keep it hidden. The anaconda strikes the house and devours one gunman and, while the other is trying to fight it off, Amanda and Scott escape. Jackie corners them, but is taken by surprise by Scott. The snake attacks Jackie and he accidentally blows himself up with a grenade while trying to destroy the snake. Scott sacrifices himself to the snake to buy time for Amanda to run. In an attempt to destroy the snake, Amanda throws a gasoline tank at the snake and blows it up, but the snake regenerates after she flees.Д«ack at the base camp, Murdoch appears, but is in for a rude shock when he sees that his arch enemy, the 'officer' who had originally accompanied Amanda earlier, Vasile. Murdoch orders Eugene to 'clean house' however it turns out Vasile offered more money and gets Eugene to join forces with him instead. When Vasile orders him to kill Murdoch, Jackson takes the moment of hostility to stab Eugene whose gun accidentally goes off and kills Vastile.

Anaconda 2 movie cast